Centro de Investigaciones Biológicas
Madrid, Spain
Description
The PhD candidate will be involved in a multidisciplinary project within the molecular recognition field. In particular, he/she will focus on the applications of state-of-the-art NMR methods to investigate biomolecular conformation, structure, and dynamics, of a variety of ligands, as well as on the specific molecular recognition events that take place between them with specific receptors. During the last few years, the group has determined the solution 3D structure of different proteins and their complexes using a protocol based on NMR spectroscopy, assisted by molecular mechanics and dynamics calculations. This methodology will be applied to different molecular complexes and to the elucidation of the structure of protein receptors of biomedical interest. The Ph D student will perform NMR studies to study the conformation and dynamics of a variety of ligands, especially carbohydrates and glycomimetics, but also from other chemical nature. Then, the interaction with different receptors will be performed using a combination of NMR methods assisted by molecular modeling protocols (docking and molecular dynamics). In particular, the interaction studies will be performed from both perspectives. From the ligand point of view and from the receptor point of view, employing modern NMR technologies: STD, Waterlogsy, and their variants. Further developments: TR-NOESY, Inter-ligand NOEs, INPHARMA, Diffusion-based methods, Paramagnetic tags: spin labels. TINS, DIRECTION, Employment of other nuclei (19F), Editing andfiltering methods. SAR by NMR. CIB-CSIC has in-house facilities for high level NMR and modeling studies. The candidate will work in strong contact with other groups of the GLYCOPHARM consortium specialized in synthesis, molecular biology, and application of other biophysical techniques.
Requirements
At the time of recruitment, applicants must be in the first four years (full-time equivalent) of their research careers, have not yet been awarded a doctoral degree, and must not have resided or carried out their main activity (work, studies, etc) in Spain for more than 12 months in the 3 years immediately prior to the reference date.
More information and applications
Application e-mail: jjbarbero@cib.csic.es
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